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Home Projects 2nd call (2009) 6. Ultrahigh resolution 3D optical imaging of live cell dynamics: application to the dynamics of the mitotic spindle and neuronal plasticity

6. Ultrahigh resolution 3D optical imaging of live cell dynamics: application to the dynamics of the mitotic spindle and neuronal plasticity

Dr. C. Depeursinge (EPFL), Dr. Y. Barral (ETHZ), Dr. P. Magistretti (EPFl) - PhD student: Etienne Shaffer

Project finished in September 2011.

Optical microscopy holds a prominent position in cell biology studies. In this grant application, we propose an innovative imaging technique based on Digital Holographic Microscopy (DHM) and nonlinear optics that is deemed to have a major impact on biomedical research in the field of cell morphological studies.

The motivation being that the relationship between cell morphology (volume and shape), shape of cellular membrane and structuring proteins (supramolecular assemblies, microtubules, actin and cytoskeleton) play a central role in biological events. The main interest of such investigations resides in the fact that they can be carried out in living cells in real time, down to the millisecond range and with a very high, nanoscale image resolution. Therefore, ultrahigh resolution dynamical studies are targeted in this proposal and aimed at the investigation of biological events.

To achieve nanoscale resolution, as well as high selectivity and functionality, we propose to combine DHM with nonlinear optics. The result, a high resolution, quantitative phase imaging technique of nonlinear optical fields would provide the required specificity for investigation of biological events, and could even be combined with other microscopy techniques, optical or not.

Contact: Etienne Shaffer

ABSTRACT NCCBI MEETING 2010

ABSTRACT NCCBI MEETING 2011

 
Home Projects 2nd call (2009) 6. Ultrahigh resolution 3D optical imaging of live cell dynamics: application to the dynamics of the mitotic spindle and neuronal plasticity